Applied Blue Biotechnology Master

Students will learn to optimize and transfer assays from in vitro to ex vivo and in vivo.

Introduction: assays in blue biotechnology, IC50 definition, threshold, controls.
Student will first learn how to perform various assays: using reactions, enzymes (measurement of inhibition or increase of activity), organites, cells (eukaryotes, prokaryotes), tissues, animals, humans.
Part 1: Target of an assay: what kind of biological activity can be assessed for human nutrition and health (metabolic syndrome (dyslipidemia, arterial hypertension, obesity, diabetes) ) cancer (angiogenesis, cell cancerization, apoptosis), skin health, ageing, oxidative stress.
Part 2: Target of an assay in the aquaculture domain,
growth parameters, mortality, color, digestive activity, reproduction, welfare indicators.

English

C2-279331-BIOT

Students will learn to design new assays to test and identify new biological activities.

Braistorm of biological activities lacking assays.
Applications focused on human health and aquaculture
Students will also learn about how to improve bioavailability and the metabolism of molecules in living organisms

English

C2-279332-BIOT

Students will conduct assays and process data.

Part 1: Methods for handling MNPs
Extraction and fractionation
Research-scale extraction and fractionation methods
Industrial-scale extraction and fractionation methods
Analysis of MNPs
HPLC
Mass spectrometry
Advanced topics: elicitation, biological engineering, co-culturing
Elicitation
Biological engineering
Co-culturing

Part 2: Relevance/performance of assays in blue biotechnology , economical evaluation/cost calculation of assays

Part 3: Practice
Sampling and extraction of marine organism.
Antimicrobial (cell scale) and antihypertensive assays (enzyme scale) will be performed.
The angiotensin I converting enzyme, which is a key enzyme in the control of arterial hypertension, will be used as a model all along the practical course. Various substrates (natural, synthetic), enzyme sources (human, animal, extracts) and analytical material (spectrophotometer, spectrofluorimeter, RP-HPLC) will be tested to understand how far such parameters can modify results of the test. Pure known molecules as well as various extracts and hydrolysates will be tested. Data obtained will be statistically analysed.

English

C2-279333-BIOT

Students will learn how to collect, isolate and identify marine organisms (microorganisms, fish, algae, etc.)

During this course, student will be able to:
Provide an overview of the biological diversity of the organisms that prosper in our oceans, including viruses, prokaryotes, microbial eukaryotes and larger organisms;
Demonstrate understanding for how our unique marine genetic resources can be a rich source of valuable products and processes to be applied to various market opportunities;
Describe some of the approaches taken to collect, identify and harness the potential of the organisms in our seas.

English

C2-279341-BIOT

Students will acquire an overview of all culture systems developed for aquaculture and will learn how to use skillfully culture systems of marine organisms (microorganisms, fish, algae, etc.) in compliance with the relevant safety and environmental requirements_Practical courses will allow student to experience marine organism culture plan, develop and innovate in different kind of marine organism cultures, including scaling-up.

English

C2-279342-BIOT

Students will become familiar with the breeding strategies of various species and will be able to develop captive-bred species development programs (fish, etc.).

At the end of this module, students will be able to:
Appreciate the role of blue biotechnology in addressing ongoing and future challenges in the culture of marine organisms to meet global demand for nutritious food; Describe specific challenges driving innovation in aquaculture, particularly around diseases, genetic improvement and the feed requirements of aquatic organisms;
Explain some of the broader considerations around legal, social and environmental acceptability when collecting and culturing marine organisms.

English

C2-279343-BIOT

Students will practice at least two other languages than English and mother tongue to be able to integrate a blue biotechnology team in Europe. Students will learn to convey ideas through a range of media.

English

DC-279301-COM

Students will develop a background knowledge of some of the problems that marine biotechnology can address;_Students will have an appreciation for commercialization and entrepreneurship and the roles that these play in marine biotechnology. They will also know the rules of intellectual properties._They will learn how to apply for a job opportunity in a foreign country.

Students will learn:
How to run a company
Entrepreneurship
Product development
Business game
Intellectual property
How to Apply for a job opportunity in foreign countries

English

C2-279302-ODP

Students will budget a biotechnological process, a project, contrast and validate results obtained in a project. They will learn how to write a research grant application and report, as well as how to present a project adequately.

English

C2-279303-ODP

Students will acquire an in-depth knowledge of the metabolites content of marine organisms and will learn how to improve macromolecules bioavailability.

Marine bioresources and marine natural products
The oceans as our last biodiversity frontier
Biodiversity and chemodiversity
Bioprospection and the Nagoya Protocol
Chemically-rich marine organisms
Actinobacteria
Cyanobacteria
Microalgae
Macroalgae
Invertebrates
Structural classes of MNPs (and their biosynthesis)
Lipids
Peptides
Terpenes
Alkaloids
Hybrids
Other

English

C2-279311-BIOT

Students will acquire an understanding of the metabolism of molecules in marine organisms.

Bioactivities :

Antibacterial
Antifungal
Antimalarial
Anti-inflammatory
Anti-ageing
Anti-obesity
Pain relief
Other

Biotechnologically- and ecologically-relevant MNP bioactivities Antifouling
Cosmetic
Nutraceutical / Nutritional
Research tools
Monitored toxins
Emergent toxins

Biotechnological processes and Marine Natural Products

English

C2-279312-BIOT

Students will acquire knowledge of specific needs for human population (elderly, sportsmen) and will learn how to integrate marine molecules in innovative products to correct physiological dysfunctions

Overview (discovery, development and supply issues)
Case studies: conopeptides, MAAs, Seattle Genetics
SME¿s conferences

English

C2-279313-BIOT

Students will work on understanding the principles governing `omics technologies : genomics, metagenomics, transcriptomics, proteomics and metabolomics, and will perform the basics of a data analysis workflow for `Omics expression data.

Introduction to Omics
Genomics: genome structure and organization, Next Generation Sequencing (NGS) techniques for genome sequencing.
Marine Metagenomics
Global analysis of gene expression and transcriptome profiling: qRT-PCR, microarrays, RNA-seq.
Introduction to Systems Biology: approaches and metabolic network analysis; network modelling.
Proteomics workflow: experimental design, sample preparation, data acquisition and data analysis. Differential expression proteomics and protein interaction approaches.

English

C2-279321-BIOT

Students will use `Omics approaches to explore microbial communities and to explore biotechnological benefits of marine ecosystems

Bioinformatics databases and sequence alignment tools
Introduction to molecular evolution and phylogeny
Basic computational skills for genomic and metagenomic analysis
Protein databases and modelling

English

C2-279322-BIOT

Student will practice `Omics to increase their laboratory skills.

After the completion of this course, students will be able to perform several fundamental laboratory techniques used for Genomics and Proteomics analyses, such as Next Generation Sequencing library preparation and 2-D electrophoresis analysis.

English

C2-279323-BIOT

During this work place base training, students will experience one or more research project(s) dealing with blue biotechnology.

English

C2-279411-STAG