Applied Blue Biotechnology Master

During this course, student will learn how to propose and optimise assays

Introduction: assays in blue biotechnology, IC50 definition, threshold, controls.
Student will first learn how to perform various assays: using reactions, enzymes (measurement of inhibition or increase of activity), organites, cells (eukaryotes, prokaryotes), tissues, animals, humans.
Part 1: Target of an assay: what kind of biological activity can be assessed for human nutrition and health (metabolic syndrome (dyslipidemia, arterial hypertension, obesity, diabetes) ) cancer (angiogenesis, cell cancerization, apoptosis), skin health, ageing, oxidative stress.
Part 2: Target of an assay in the aquaculture domain,
growth parameters, mortality, color, digestive activity, reproduction, welfare indicators.

English

C2-279331-BIOT

During this course, student will learn how to propose novel assays to identify new biological activities.

Braistorm of biological activities lacking assays.
Applications focused on human health and aquaculture
Students will also learn about how to improve bioavailability and the metabolism of molecules in living organisms

English

C2-279332-BIOT

During this course, student will learn about methods to handle MNPs, perform some assays and treat data obtained from such assays

Part 1: Methods for handling MNPs
Extraction and fractionation
Research-scale extraction and fractionation methods
Industrial-scale extraction and fractionation methods
Analysis of MNPs
HPLC
Mass spectrometry
Advanced topics: elicitation, biological engineering, co-culturing
Elicitation
Biological engineering
Co-culturing

Part 2: Relevance/performance of assays in blue biotechnology , economical evaluation/cost calculation of assays

Part 3: Practice
Sampling and extraction of marine organism.
Antimicrobial (cell scale) and antihypertensive assays (enzyme scale) will be performed.
The angiotensin I converting enzyme, which is a key enzyme in the control of arterial hypertension, will be used as a model all along the practical course. Various substrates (natural, synthetic), enzyme sources (human, animal, extracts) and analytical material (spectrophotometer, spectrofluorimeter, RP-HPLC) will be tested to understand how far such parameters can modify results of the test. Pure known molecules as well as various extracts and hydrolysates will be tested. Data obtained will be statistically analysed.

English

C2-279333-BIOT

During this course, student will be able to:
Provide an overview of the biological diversity of the organisms that prosper in our oceans, including viruses, prokaryotes, microbial eukaryotes and larger organisms;
Demonstrate understanding for how our unique marine genetic resources can be a rich source of valuable products and processes to be applied to various market opportunities;
Describe some of the approaches taken to collect, identify and harness the potential of the organisms in our seas.

English

C2-279341-BIOT

At the end of this module, students will be able to:
Describe systems used to cultivate common marine organisms for food and non-food products, including algae, fish and invertebrates;
Recognise the scale and extent of aquaculture across Europe and elsewhere, including an overview of value chains and knowledge of the key challenges facing these industries.

English

C2-279342-BIOT

At the end of this module, students will be able to:
Appreciate the role of blue biotechnology in addressing ongoing and future challenges in the culture of marine organisms to meet global demand for nutritious food; Describe specific challenges driving innovation in aquaculture, particularly around diseases, genetic improvement and the feed requirements of aquatic organisms;
Explain some of the broader considerations around legal, social and environmental acceptability when collecting and culturing marine organisms.

English

C2-279343-BIOT

During this course, student will learn some words of at least two other languages than English and mother tongue to be able to integrate a blue biotechnology team in Europe. Student will learn to convey ideas through a range of media.

English

DC-279301-COM

Students will have an appreciation for commercialization and entrepreneurism and the roles that these play in marine biotechnology and achieve a basic understanding of intellectual property and its value.

Students will learn:
How to run a company
Entrepreneurship
Product development
Business game
Intellectual property
To Apply to a job opportunity in foreign country

English

C2-279302-ODP

During this course, student will budget a biotechnological process, a project, a feed formulation
contrast and validate results obtained in a project.
Working with data (types, analysis, organisation, stats)
how to develop a research grant application
Report and present in adequate manner a project
Critical reading, lab note books, records.

English

C2-279303-ODP

During this course student will acquire in-depth knowledge of the metabolites produced by marine organisms.

Marine bioresources and marine natural products
The oceans as our last biodiversity frontier
Biodiversity and chemodiversity
Bioprospection and the Nagoya Protocol
Chemically-rich marine organisms
Actinobacteria
Cyanobacteria
Microalgae
Macroalgae
Invertebrates
Structural classes of MNPs (and their biosynthesis)
Lipids
Peptides
Terpenes
Alkaloids
Hybrids
Other

English

C2-279311-BIOT

During this course, students will study showcase of pharmacologically-relevant MNP bioactivities.

Bioactivities :

Antibacterial
Antifungal
Antimalarial
Anti-inflammatory
Anti-ageing
Anti-obesity
Pain relief
Other

Biotechnologically- and ecologically-relevant MNP bioactivities Antifouling
Cosmetic
Nutraceutical / Nutritional
Research tools
Monitored toxins
Emergent toxins

Biotechnological processes and Marine Natural Products

English

C2-279312-BIOT

During this course, student will study cases with teachers and professionals.

Overview (discovery, development and supply issues)
Case studies: conopeptides, MAAs, Seattle Genetics
SME¿s conferences

English

C2-279313-BIOT

During this course, student will gain insight into the principles governing ¿Omics¿ approaches: Genomics, Metagenomics, Transcriptomics, Proteomics and Metabolomics, learning the basic experimental and data analysis workflows for these domains.

Introduction to Omics
Genomics: genome structure and organization, Next Generation Sequencing (NGS) techniques for genome sequencing.
Marine Metagenomics
Global analysis of gene expression and transcriptome profiling: qRT-PCR, microarrays, RNA-seq.
Introduction to Systems Biology: approaches and metabolic network analysis; network modelling.
Proteomics workflow: experimental design, sample preparation, data acquisition and data analysis. Differential expression proteomics and protein interaction approaches.

English

C2-279321-BIOT

Students will acquire the computational tools needed to be able to study biological systems, as well as the use on online databases to access to the relevant biological data.

Bioinformatics databases and sequence alignment tools
Introduction to molecular evolution and phylogeny
Basic computational skills for genomic and metagenomic analysis
Protein databases and modelling

English

C2-279322-BIOT

After the completion of this course, students will be able to perform several fundamental laboratory techniques used for Genomics and Proteomics analyses, such as Next Generation Sequencing library preparation and 2-D electrophoresis analysis.

Genomics: Next Generation Sequencing library preparation, quality control and validation.
Basic proteomics laboratory: protein 2-D electrophoresis.

English

C2-279323-BIOT

During this work place base training, students will experience one or more research project(s) dealing with blue biotechnology.

English

C2-279411-STAG